Notebook
You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very ustoeful organizational tool as well.
| July |
| Sun. | Mon. | Tues. | Wed. | Thurs. | Fri. | Sat. |
|
| 1 | 2 | 3 | 4 | 5 |
| 6 | 7 | 8 | 9 | 10 | 11 | 12 |
| 13 | 14 | 15 | 16 | 17 | 18 | 19 |
| 20 | 21 | 22 | 23 | 24 | 25 | 26 |
| 27 | 28 | 29 | 30 | 31 |
|
|
|
| August |
| Sun. | Mon. | Tues. | Wed. | Thurs. | Fri. | Sat. |
|
|
|
|
| 1 | 2 |
| 3 | 4 | 5 | 6 | 7 | 8 | 9 |
| 10 | 11 | 12 | 13 | 14 | 15 | 16 |
| 17 | 18 | 19 | 20 | 21 | 22 | 23 |
| 24 | 25 | 26 | 27 | 28 | 29 | 30 |
| 31 |
|
|
|
|
|
|
|
| September |
| Sun. | Mon. | Tues. | Wed. | Thurs. | Fri. | Sat. |
| 1 | 2 | 3 | 4 | 5 | 6 |
| 7 | 8 | 9 | 10 | 11 | 12 | 13 |
| 14 | 15 | 16 | 17 | 18 | 19 | 20 |
| 21 | 22 | 23 | 24 | 25 | 26 | 27 |
| 28 | 29 | 30 |
|
|
|
|
|
|---|
7/15
transformation
| Name | Sample(µl) | Competent Cells(µl) | Total(µl) | Plate |
|---|
| BBa_J23101 | 2 | 20(2/23) | 22 | LB（CP） |
| BBA_B0032 | 2 | 20（2/23） | 22 | LB（CP） |
7/16
transformation
| Name | Sample(µl) | Competent Cells(µl) | Total(µl) | Plate |
|---|
| BBa_B0030 | 2 | 20(2/23) | 22 | LB（CP） |
| BBA_B0032 | 2 | 20（2/23） | 22 | LB（CP） |
7/17
Colony PCR
| Sample |
|---|
| 7/16 BBa_B0030-(1) |
| 7/16 BBa_B0030-(2) |
| 7/16 BBa_B0030-(3) |
| 7/16 BBa_B0030-(4) |
| 7/16 BBa_B0030-(5) |
| 7/16 BBa_B0030-(6) |
| 7/16 BBa_B0032-(1) |
| 7/16 BBa_B0032-(2) |
| 7/16 BBa_B0032-(3) |
| 7/16 BBa_B0032-(4) |
| 7/16 BBa_B0032-(5) |
| 7/16 BBa_B0032-(6) |
| NC |
Liquid Culture
| Sample | medium |
|---|
| 7/16 BBa_B0030-(1) | Plusgrow medium(+CP) |
| 7/16 BBa_B0030-(2) | Plusgrow medium(+CP) |
| 7/16 BBa_B0030-(3) | Plusgrow medium(+CP) |
| 7/16 BBa_B0030-(4) | Plusgrow medium(+CP) |
| 7/16 BBa_B0030-(5) | Plusgrow medium(+CP) |
| 7/16 BBa_B0030-(6) | Plusgrow medium(+CP) |
| 7/16 BBa_B0032-(1) | Plusgrow medium(+CP) |
| 7/16 BBa_B0032-(2) | Plusgrow medium(+CP) |
| 7/16 BBa_B0032-(3) | Plusgrow medium(+CP) |
| 7/16 BBa_B0032-(4) | Plusgrow medium(+CP) |
| 7/16 BBa_B0032-(5) | Plusgrow medium(+CP) |
| 7/16 BBa_B0032-(6) | Plusgrow medium(+CP) |
7/18
Miniprep
| DNA | Concentration(µg/ml) | 260/280 | 260/230 |
|---|
| 7/16 BBa_B0030-(1) | 229 | 1.96 | 1.40 |
| 7/16 BBa_B0030-(2) | 209 | 1.95 | 1.50 |
| 7/16 BBa_B0030-(3) | 108 | 2.12 | 2.12 |
| 7/16 BBa_B0030-(4) | 186 | - | - |
| 7/16 BBa_B0030-(5) | 174 | 2.02 | 1.56 |
| 7/16 BBa_B0030-(6) | 158 | 2.01 | 1.68 |
| 7/16 BBa_B0032-(1) | 179 | 2.00 | 2.06 |
| 7/16 BBa_B0032-(3) | 221 | 1.90 | 1.37 |
| 7/16 BBa_B0032-(4) | 225 | 1.89 | 1.37 |
| 7/16 BBa_B0032-(5) | 270 | 1.98 | 1.63 |
| 7/16 BBa_B0032-(6) | 194 | 1.89 | 1.33 |
7/28
Restriction Enzyme Digestion
| 7/18 RBS Sample | EcoR1 | Xba1 | Spe1 | Pst1 | Buffur | BSA | MilliQ | TOTAL |
|---|
| Sample1 | 0.3 | 0 | 0 | 0 | 1(H) | 0 | 7.7 | 10 |
| Sample2 | 0 | 0.3 | 0 | 0 | 1(M) | 1 | 6.7 | 10 |
| Sample3 | 0 | 0 | 0.3 | 0 | 1(M) | 0 | 7.7 | 10 |
| Sample4 | 0 | 0 | 0 | 0.3 | 1(H) | 0 | 7.7 | 10 |
| Control | 0 | 0 | 0 | 0 | 1 | 0 | 8 | 10 |
"
