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From 2014.igem.org

Contents 1 Friday 1st August 1.1 Lab work 1.1.1 A - The frame coli Odor free 1.1.1.1 Stria on LB dishes 1.1.2 C - Salicylate Inducible Suppressing System 1.1.2.1 Liquid Culture 1.1.3 D - Lemon scent 1.1.3.1 Results of the Transformation of electrocompetent cells

Friday 1st August

Lab work

A - The frame coli Odor free

Stria on LB dishes

by Romain

Strains used: MG1655 and MG1655Z1, striated on 4 LB dishes per strain.

C - Salicylate Inducible Suppressing System

Liquid Culture

by Fabio

8 liquid cultures with 3ml of LB and 3µl of amp (at 10am - 8°C), all from the colonies made the 31st July. The colonies will be put at 37°C on sunday 3rd in order to have grown colonies on monday.

D - Lemon scent

Results of the Transformation of electrocompetent cells

by Arnaud & Terry

Transformation of electrocompetent cells made the July 29th. Strains used: DY330. Plasmid used: pJBEI-6409 (code for enzymes to produce limonene from glucose in E. coli)

Results:

• Nothing has grown on the control dishes.
• Failure, nothing has grown on the dishes which contain DY330 with pJBE1

Members there:

• Instructors and advisors: Alice, Solenne and Sylvie.
• Students: Arnaud, Fabio, Romain, Sean and Terry.

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