Team:Paris Saclay/Protocols

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Protocols

  1. Preparation of supercompetent E.coli cells
  2. Transformations of supercompetent E. coli cells
  3. Extraction of the Genomic DNA from Bacteria by using NucleoSpin® Tissue
  4. Extraction of the Genomic DNA from Bacteria without NucleoSpin® Tissue
  5. Gel Electrophoresis
  6. Transformation of CaCl2 supercompetent E.coli cells
  7. PCR for the genomic DNA of bacteria
  8. PCR clean-up
  9. PCR for bacterial culture
  10. Transformation of competent cells by electroporation
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