Team:Brasil-SP/Project/ResponseModule

Blood sample from a person with low levels of circulating Cystatin C triggers the expression of the reporter gene GFP in the biodetector, resulting in fluorescent signals. On the other hand, blood sample with high levels of Cystatin C does not induce the production of GFP, resulting in absent of fluorescence. Our test is carried out with patients plasma (without blood cells) filtered through a microfluidics device. In this device, our modified Bacillus subtilis will stored as spores, which are activated by rich medium (Luria-Bertani [LB]) before being used in the device. In order to induce the spore formation, the bacteria are incubated in a minimal nutrient medium (Difco Sporulation Medium [DSM]).