Team:Cambridge-JIC/Constructs/gal4 hap1gr H2RBmRFP

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Gal4 - Hap1GR - H2BmRFP

Design

Aim of the construct

  • We'll try out 35s->GAL4->HAP1GR->H2BmRFP.
  • Since H2BmRFP has not been tried in Mp, we'll also try 35s->H2BmRFP

End goal plasmid

Experimentation (up to date)

PCR

Attempt 1:

Gibson

Attempt 1:
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E-Coli transformation

Attempt 1:
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Agrobacteria transformation

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Spore preparation

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Spore transformation

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Evaluation

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