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From 2014.igem.org

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Informatics

To introduce a novel chassis and make full advantage of its properties, we want to computationally analyse its genome in order to:

• optimise codon usage in our registry parts and to facilitate future synthetic biology work on Marchantia;
• identify and characterise promoters, in particular looking for strong, inducible, tissue-specific or early development stage promoters.

Codon usage optimisation

Our start point was the Marchantia genome and the Open Reading Frame (ORF) predicted with the Geneious software (http://www.geneious.com/).