Frozen Cell Stock Preparation

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1. Grow a liquid cell culture of the strain to be made competent overnight.
   
   
2. Pipette 0.666ml of liquid cell culture with 0.333ml of 60% glycerol (to give an overall percentage glycerol 20% and total volume of 1ml) into an eppendorf tube.
   
   
3. Put into the freezer at -80°C.