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From 2014.igem.org

Contents 1 Thursday 17th July 1.1 Lab work 1.1.1 1 - Preparation of supercompetent cells 1.1.2 2 - Bacterial Culture 1.1.3 3 - PCR and Electrophoresis

Thursday 17th July

Lab work

1 - Preparation of supercompetent cells

By Mathias & Romain

Strains used: MG1655 (x2), MG1655Z1 (x2).

Protocol

2 - Bacterial Culture

by Fabio and Maher

2 liquid cultures with 5ml of LB and 5µl of amp (at 9am - 37°C - 150 rpm), both J23119 from the concentrated colony made the 16th July

3 - PCR and Electrophoresis

by Arnaud & Mathias

PCR targeting with oligos IPS70 and IPS71 of pOsV230 (Apramycine).

1. Molecular weight marker 1kb
2. Empty
3. IPS70 and IPS71 (1)
4. IPS70 and IPS71 (2)

Results:

Failure, no PCR products.

Protocol

People there:

• Instructors and advisors: Alice and Solenne.
• Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.

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