Team:Cambridge-JIC/Dry Work

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Constructs

  • Tip 1
    • Delete 5'-UTRs (if in doubt). 5'-UTR's affect expression rates, so if we're comparing promoters/inputs, UTR's will make a big difference. So, for the sake of normalisation, get rid of the 5'-UTRs.
  • Tip 2
    • If it works, don't change it. Don't go altering spacing in between parts when making constructs!

Primers

  • Tip 1
    • Make the primers. Don't really worry about secondary structures unless it's obvious. Use a melting temperature of >58 degrees.
  • Tip 2
    • Check that they are correct by searching in the plasmid you're PCR'ing off and the destination construct.

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