Team:Cambridge-JIC/Protocol
From 2014.igem.org
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General outline of the method:
- Polymerase Chain Reaction (PCR) to obtain DNA fragments
- Gel electrophoresis to select the correct fragments
- DNA purification for plasmid assembly
- Gibson Assembly
- E. coli transformation and plating
- Colony analysis and selection by colony PCR
- Miniprep and sequencing