Team:Evry/Notebook/Sensing/Phenol/09-18-2014
From 2014.igem.org
Assembling of the parts
Ligation of all parts
Transformation on E.coli
Purification of palsmids with NucleoSpin Plamsid Kit (Macherey Nagel) on 4 colonies
Digestion with EcoRI
DIGEST E GFP32DMPR C1-C2-C3-PARENT C4