Team:Paris Bettencourt/Results

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Smell
the roses		 Teen
spirit		 Something
fishy		 Goody
two shoes		 Don't
sweat it



Smell The Roses

  • Created BioBricks coding for sequences for different enzymes to nullify the bad odor produced by E. coli.
  • We produced the smells that compose the main odor categories perceived by humans.
  • BioBricks submitted to be BioBrick registry:BBa_K1403003, BBa_K1403006, BBa_K1403009, BBa_K1403012, BBa_K1403017, BBa_K1403019

    • Teen Spirit

    ‘

    • Isolated 4 bacterial species capable of growing on 2-nonenal as a carbon source.
    • Determined the critical smell detection limit of 2-nonenal.
    • Analyzed 2-nonenal tolerance and degradation in natural isolates.

    Something Fishy

    • Cloned tmm into E.coli using pSB1C3 and pSEVA315 vectors, creating a new Biobrick: BBa_K1403015.
    • Characterized and quantified the activity of TMM by a colorimetric assay.
    • Confirmed the degradation of trimethylamine by gas chromatography - mass spectrometry (GC/MS).
    .

    Goody Two Shoes

  • Demonstrated that mutant strains of B. subtilis produce less odor.
  • Measured growth and viability of mutants in synthetic sweat media and on socks.

    • Don't Sweat It

  • Expressed the odor gene agaA in E. coli (BBa_K1403002).
  • Designed and used CRISPRs to enrich for loss-of-function mutants 1000x in a mixed population.
  • Identified potential CRISPR targets as odor genes with variability hotspots in metagenome data.
  • Formulated a simple skin cream encorporating live bacteria.

    • Interlab study

    The aim of the Interlab Study was to measure GFP expression under different Anderson promoters and in different plasmids.
    iGEM Paris Bettencourt team

    • - Successfully transformed E.coli NEB with BBa_I20260 (J23101-B0032-E0040-B0010-B0012) in the pSB3K3 vector - Device 1
    • - Successfully built BBa_J23101 + BBa_E0240 (B0032-E0040-B0015) in pSB1C3 backbone - Device 2
    • - Successfully built BBa_J23115 + BBa_E0240 (B0032-E0040-B0015) in pSB1C3 backbone - Device 3
    • - Sequenced and gel verified the three devices
    • - Characterized all the three devices with the OD600 and fluorescence measurements in a Tecan micro-plate reader
    • - Reported the results on the Interlab Study page of the iGEM wiki
    • - Completed the description of the BBa_I20260 - Device 1
    • - Submitted and send parts: BBa_K1403000 - Device 2 and BBa_K1403001 - Device 3 to the Biobricks registry

    Collaborations

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    Centre for Research and Interdisciplinarity (CRI)
    Faculty of Medicine Cochin Port-Royal, South wing, 2nd floor
    Paris Descartes University
    24, rue du Faubourg Saint Jacques
    75014 Paris, France
    +33 1 44 41 25 22/25
    paris-bettencourt-igem@googlegroups.com
    Copyright (c) 2014 igem.org. All rights reserved.

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