"

From 2014.igem.org

BYU 2014 Notebook EDIT Procedures

Home Team Official Team Profile Project Parts Modeling Notebook Safety Attributions

Q5 PCR Reaction:

10 uL 5x Q5 Reaction Buffer

1 uL dNTPs

1 uL Forward Primer

1 uL Reverse Primer

10 uL Q5 Enhancer

23.5 uL ddH2O

1-2 uL Template

---------------

50 uL Reaction

uL Q5 Polymerase (add right before you start the PCR reaction. Keep on ice until you are ready to do so.)