Team:BYU Provo/Notebook/CommonProcedures

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BYU 2014 Notebook

EDIT Procedures

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Q5 PCR Reaction:

10 uL 5x Q5 Reaction Buffer

1 uL dNTPs

1 uL Forward Primer

1 uL Reverse Primer

10 uL Q5 Enhancer

23.5 uL ddH2O

1-2 uL Template

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50 uL Reaction



uL Q5 Polymerase (add right before you start the PCR reaction. Keep on ice until you are ready to do so.)