Team:UFMG Brazil/Project/Notebook

*First meeting with the team

*Possibilities of themes:
→ Metabolic syndrome,
→ Promoter sensitive to sound
→ Use of yeast
*Doodle
*Description
*Trying to get support from Pro-reitoria→ Professor. Adelina
*Sponsorship

*Defined theme:
→Use of yeast for detection and treatment of colon cancer

*Vakinha (Crowd funding)

*Defined theme:
→Use of yeast for detection and treatment of colon cancer

*Cancer of colon
*Probiotic: UFMG 905
*Biomarkers: sensors
*Human practices (Meagan)
→ Difficulties to do Science in Brazil
→Documentary
→ Buttons "I'm from Latin America"
→ Lectures

*Identification system Colon cancer (Carlos)
→Quantify the DNA present in the feces of mice with cancer (Laila)
→Kit for DNA extraction from faeces (Rodrigo);
→Disclosure TV radio and medias
→Refresh the iGEM Facebook page
→Search for new options field of non-specific DNA binding

*Long strand DNA
→Extracting DNA from faeces of mice

→System
*Chimera proteins: look at other
teams
* Importance of attendance at team
meetings
* Romario: looking press office

*Oncologists
→Situation of colon cancer
→Diagnosis
*CCSP-2 (Oncogenes 2005) → Von
Willebrand
→Search for markers
→DNA (Carlos)
→Carbohydrates (Laila / Isadora /
Lucas)
→CCSP-2 (Rodrigo and Michele)
→Metabolites (Marcelo and Dani)

*Need to find for sponsorship (Ciça
and Chris)
*Idea to elaborate questionnaire to
other Latin American iGEM teams (Thiago and Veronica)

*TALE: Specific Sequence. DNA binding
*mCherry split: NEW !!!
*Can we use FRET techniques?
*mCherry modeling
*Microsatellite
*Synthesis of template DNA repeat

*Ca-binding proteins overexpressed
++
*Biosafety
*CCSP-2: two domains von wilebrand

*Workers yeasts: central information
(more than one signal
"warning" the same cell)

*Division of the responsibilities:
→Draw the biobricks (Carlos)
→Collecting mice faeces (Laila)
→Protocol for DNA extraction from faeces and quantification (Rodrigo and
Michele)
→Human Practices (Thiago Mafra)
→Cancer in the past IGEM (Marcelo and Dani Chame)
→Video animation of IGEM UFMG BRAZIL (Lucas and Isadora)
→Sponsors (Cecilia, Chris, James and Veronica Bruno)
→Translate questionnaire to Spanish (Dani Chame)

*Conversation with Paulo Sérgio Lacerda Beirão
→Legislation of the country
*Videos with testimonials about the science in the country of researchers

*Metabolic Syndrome - network of diseases
*Biomarkers choice → limit
→Complexity
→Physiology
→Stability
*Lenght DNA→ eletricity (3’
5’)
*Accumulation of signal
*Transfer of fluorescence
*DNA bound proteins: proteins
crawling in the DNA
*Noise/signal ratio: important for
qualitative diagnosis
*Stool: noise
*Proximity can allow interaction
with domains that will activate an enzyme that transform a smelly (banana?)
substract
OR
*Smell constitutive?
*Yeast die if it dos not meet
signal.
*Difference between short and long
DNA
*NEW idea: sound detection.
*mCherry

*Protocol for feces
*Modeling (Gastone)
→Probability of false positives
*Yeast plasmids design
*Paratope Fab / Histone
*Validation in E.coli: pass after apra yeast synthesize:
→TALE
→mCherry
→Long repetitive DNA
→Short repetitive DNA

*TALE: Specific Sequence. DNA binding
*mCherry split: NEW !!!
*Can we use FRET techniques?
*mCherry modeling
*Microsatellite
*Synthesis of template DNA repeat

*Ca-binding proteins overexpressed
++
*Biosafety
*CCSP-2: two domains von wilebrand

*Workers yeasts: central information
(more than one signal
"warning" the same cell)

*Workshop Biosynth
*How to earn money?
→Brazilian companies?
→INCT
→Proreitoria
→Mario Penna

*Latin America Fan Page

*RFC10 - Biobrick standard
*Bsmb1 → TALE
*Software Assembly?
*Wiki
*Wet lab - gene synthesis
*How to earn money?

*Discount in the synthesis
*Enzymes: XbaI, SpeI, EcoR1, Pst1,
AgeI, NgomIV, BsmbI
→Phoneutria?
*Jacques Nicoli→ Yeast
*Biosafety (term 23/06)
*Donation Fiocruz

*New England Enzyme
*Texts Wiki
→Project
→Team
→Human practices
→Facebook
→Vakinha (Crowd funding)

* Experimental tests
* shopping
* Restriction enzymes
* length of 110 nucleotides linker → → try a smaller
*experimental
→Biobrick assemble the plasmid
→Sequencing
→Express and perform the tests in vitro.
→Write protocols for wiki
*Modeling mathematics
*Wiki:
→Assemble layout and content.
*Human Practices
→Questionnaires
→Sponsorships
→Questions - students ufmg schools.
*Bioethics

*Price for synthesizing biobrick
→Request assistance pro-reitoria

*Modeling
→test of interaction assemblies TALE + + Linker Hmcherry1 and 2
*Making a letter for sponsorship

*Deadlines
* Fundraising Ideas
* Human Practices
→ Send e-mails with the questionnaires
* Modeling of proteins
*Extraction of faeces

* Arrival of restriction enzyme
→ Work
*Texts for wiki
*inscription IGEM

*Experiments

*Sponsors

*Experiments
* Human Practices
→Results of the questionnaires
* Modeling of proteins

*Meeting with Dr.Clélio Campolina, National minister of
Science and Technology
*Wiki

*Human Practices: low adhesion
*Fetch data from investment in research
*Participation in Latin America over the years in the iGem
*Plot: X number of contacts feedback
*Lack of appreciation of Science
*Popular search

*Experiments
*Project Summary
*Meeting with Dr. Evaldo Vilela,
FAPEMIG director.

*Finish the Wiki
Modeling
*Experimental
*Human practices.
→Questionnaire in the 7 square

*Lecture to high school, Technical school and Graduation, at
CEFET.
* Survey: The Importance of Science
* Concentrations DNA
* Sponsors
* Deadlines

* Experiments
→Results
* Human Practices
→Results
*Modeling of proteins
→Results
* Modeling mathematics
→Results
* Sponsors
→Tshirt
→Who helped?

World Championship Jamboree.