Team:Paris Bettencourt/Results

Smell The Roses

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Teen Spirit

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• Isolated 4 bacterial species capable of growing on 2-nonenal as a carbon source.
• Determined the critical smell detection limit of 2-nonenal.
• Analyzed 2-nonenal tolerance and degradation in natural isolates.

Something Fishy

• Cloned tmm into E.coli using pSB1C3 and pSEVA315 vectors, creating a new Biobrick: BBa_K1403015.
• Characterized and quantified the activity of TMM by a colorimetric assay.
• Confirmed the degradation of trimethylamine by gas chromatography - mass spectrometry (GC/MS).

Goody Two Shoes

Don't Sweat It

- Designed a BioBrick of agaA (main gene responsible for body odor) in pSB1C3.
- Found hotspots for genes ackA and ldh (responsible for body odor) found in human microbiome samples.
- Design CRISPRs to select for natural odorless strains.
- Made a DIY formulation of probiotic cream.

Human practice

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Interlab study

The aim of the Interlab Study was to measure GFP expression under different Anderson promoters and in different plasmids.
iGEM Paris Bettencourt team

• - Successfully transformed E.coli NEB with BBa_I20260 (J23101-B0032-E0040-B0010-B0012) in the pSB3K3 vector - Device 1
• - Successfully built BBa_J23101 + BBa_E0240 (B0032-E0040-B0015) in pSB1C3 backbone - Device 2
• - Successfully built BBa_J23115 + BBa_E0240 (B0032-E0040-B0015) in pSB1C3 backbone - Device 3
• - Sequenced and gel verified the three devices
• - Characterized all the three devices with the OD600 and fluorescence measurements in a Tecan micro-plate reader
• - Reported the results on the Interlab Study page of the iGEM wiki
• - Completed the description of the BBa_I20260 - Device 1
• - Submitted and send parts: BBa_K1403000 - Device 2 and BBa_K1403001 - Device 3 to the Biobricks registry

Collaborations

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