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Team:Macquarie Australia/WetLab/Notebook
From 2014.igem.org
The Notebook
Welcome to our Notebook! This page has been created to detail our projects progress since its conception. Click on the section titles below to expand pages that contain information regarding what we worked on and achieved each week. For a more detailed summary of what we achieved please visit the Results page.
November 2013
Week 1
Biobrick stocktake of 2013 iGEM Macquarie_Australia parts: 11/11/13
- ChlG - sufficient plasmid stock
- DVR1 - sufficient plasmid stock
- ChlM - sufficient plasmid stock
- ChlI2 - need more plasmid stock
- POR- need more plasmid stock
- YCF - need more plasmid stock
- Plasto - need more plasmid stock
- GUN4- need more plasmid stock
- CTH1 - need more plasmid stock
- ChlD - need more plasmid stock. Question whether the 2013 part is really the reported sequence - something appears to be missing.
Send all for re-sequencing to verify DNA sequence as per registry entries.
DVR1 re-tranformation: Was re-done using gibson assembly and then transformed.
Week 2
Sequencing Results: all parts except ChlD were correct.ChlD Fix: ChlD is missing 50 bp. Strategy to correct is to use ApaI and MluI restriction enzymes to cut out 50bp from clone of ChlD in pET vector from Willows group and re-insert into our BioBrick vector.
December 2013
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January 2014
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February 2014
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August 2014 - Week 1
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