Team:Penn/Protocol

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University of Pennsylvania iGEM

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Protocols

E.Coli Specific Protocols

Restriction Enzyme Digest

Ligation

Transformation

Innoculation

AMB-1 Specific Protocols

Our protocols for working with AMB-1 have been outlined below. However, for easy access to consolidated, optimized protocols, refer to the AMB-1 Specification Sheet we developed.

Click here to view the Spec Sheet!

Glycerol Stock

MSGM Media Preparation

EMSGM Media Preparation

Inoculation of Aerobic Cultures

Counting Cell Cultures

Transformation Protocol 1

Transformation Protocol 2

Penn iGEM Transformation Protocol

Plating Techniques

Materials and Methods

Miniprep

Determining Correlation between OD600 and Cell Concentration for Aerobic and Anaerobic Cultures

Optimizing Transformation

Gel Extraction