Protocols

• PCR
Instruction for colony PCR and regular PCR


• Gel Verification
Make a gel and conduct gel elecrophoresis


• BioBrick Restriction Digest
Restriction digest with BioBrick plasmid an inserts


• BioBrick Ligation
Ligate Biobrick insert to vector


• Making Plates
Make LB plates with antibiotics


• Liquid media
Make liquid LB media


• Competent cells
Preparation of chemically competent cells


• Transformation
Transformation of plasmi DNA into chemically competent cells


• CRISPR Repeat-Spacer Array Assembly
Assemble combinatorial libraries of repeat-spacer-repeat arrays using oligonucleotides


• Assembling Parts from Oligonucleotides
Assemble short parts from annealed oligonucleotides


• (Multi) Site Directed Mutagenesis
Site directed mutagenesis. Protocol is directly amenable simultaneously making distant mutations.


• Oligonucleotide Phosphorylation
Phosphorylate oligonucleotides prior to ligation


• Electrocompetent Cell Production


v Make electrocompetent cells
• Make Combinatorial Part Libraries
Make combinatorial part libraries from compatible parts