Protocols

• PCR
Instruction for colony PCR and regular PCR


• Gel Verification
Make a gel and conduct gel elecrophoresis

BioBrick Restriction Digest

Restriction digest with BioBrick plasmid an inserts

===[[Team:British_Columbia/Notebook/Protocols/ligation|BioBrick Ligation]]=== Ligate Biobrick insert to vector ===[[Team:British_Columbia/Notebook/Protocols/plates|Making Plates]]=== Make LB plates with antibiotics ===[[Team:British_Columbia/Notebook/Protocols/media|Liquid media]]=== Make liquid LB media ===[[Team:British_Columbia/Notebook/Protocols/compcells|Competent cells]]=== Preparation of chemically competent cells ===[[Team:British_Columbia/Notebook/Protocols/transformation|Transformation]]=== Transformation of plasmi DNA into chemically competent cells ===[[Team:British_Columbia/Notebook/Protocols/RepeatSpacerAssembly |CRISPR Repeat-Spacer Array Assembly]]=== Assemble combinatorial libraries of repeat-spacer-repeat arrays using oligonucleotides ===[[Team:British_Columbia/Notebook/Protocols/AnnealedOligonucleotides|Assembling Parts from Oligonucleotides]]=== Assemble short parts from annealed oligonucleotides ===[[Team:British_Columbia/Notebook/Protocols/MultisiteMutagenesis|(Multi) Site Directed Mutagenesis]]=== Site directed mutagenesis. Protocol is directly amenable simultaneously making distant mutations. ===[[Team:British_Columbia/Notebook/Protocols/T4PNK|Oligonucleotide Phosphorylation]]=== Phosphorylate oligonucleotides prior to ligation ===[[Team:British_Columbia/Notebook/Protocols/ElectrocompCells|Electrocompetent Cell Production]]=== Make electrocompetent cells ===[[Team:British_Columbia/Notebook/Protocols/CombinatorialLibraries|Make Combinatorial Part Libraries]]=== Make combinatorial part libraries from compatible parts