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Team:Oxford/modellingA
From 2014.igem.org
[DCM] calibration for solution-vapour equilibration
Testing the tolerance of E-coli and Pseudomonas strains to various concentrations of DCM (0mM, 5mM, 10mM, 20mM), the 15 mL falcon tube-contained system is modeled as a binary solution/ideal gas, based on literature solubility data, confirming negligible [DCM] deviation:
|
|
p(DCM) /kPa |
n(g) /mol |
V(DCM) added /ml |
n(DCM)(l) unadjusted /mol |
[DCM] unadjusted /M |
n(DCM)(l) adjusted /mol |
[DCM] adjusted /M |
[DCM] % deviation |
|
(30°C, 5mM) |
1.56E-04 |
3.94E-12 |
6.4E-03 |
1.0E-04 |
2.0E-02 |
1.0E-04 |
2.0E-02 |
3.9E-06 |
|
(30°C, 10mM) |
3.12E-04 |
7.88E-12 |
3.2E-03 |
5.0E-05 |
1.0E-02 |
5.0E-05 |
1.0E-02 |
1.6E-05 |
|
(30°C, 20mM) |
6.24E-04 |
1.58E-11 |
1.6E-03 |
2.5E-05 |
5.0E-03 |
2.5E-05 |
5.0E-03 |
6.3E-05 |
|
|
|
|
|
|
|
|
|
|
|
(37°C, 5mM) |
1.56E-04 |
3.94E-12 |
6.4E-03 |
1.0E-04 |
2.0E-02 |
1.0E-04 |
2.0E-02 |
3.9E-06 |
|
(37°C, 10mM) |
3.12E-04 |
7.88E-12 |
3.2E-03 |
5.0E-05 |
1.0E-02 |
5.0E-05 |
1.0E-02 |
1.6E-05 |
|
(37°C, 20mM) |
6.24E-04 |
1.58E-11 |
1.6E-03 |
2.5E-05 |
5.0E-03 |
2.5E-05 |
5.0E-03 |
6.3E-05 |
