Team:Groningen/Template/MODULE/projects/parts/secretion

Infection by Staphylococcus aureus and Pseudomonas aeruginosa will be battled by Lactococcus lactis using molecules that prevent biofilm formation, disrupt communication between pathogens and kill the pathogen.

When bacteria start infection of a wound, they often protect themselves with a biofilm. For an effective fight against these infections, the biofilm should be broken down. We have assembled a BioBrick of dispersinB fused to an USP45tag. This part was later assembled in the secretion system, and can work against S. aureus and P. aeruginosa .

We will fight against P. aeruginosa with an dual action system, consisting of the new BioBricks dispersinB and aiiA. DispersinB works against biofilm formation as described above. AiiA is an quorum quenching enzyme which disrupt communication between P. aeruginosa. By disrupting communication we try reduce the activity of both dividing and activation of pathogenic genes by P. aeruginosa.²

The cell wall of the Gram-positive bacterium S. aureus contains Lipid-2 fatty acids. These are perfect targets for the anti-microbial nisin.³ We designed and assembled nisin in an a novel BioBrick that combines the expression of dispersinB and nisA. With this second dual action system, we want to fight of infections by S. aureus.