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Team:Groningen/Template/MODULE/Notebook/vector/week1
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Revision as of 20:04, 17 October 2014 by Lisahielkema (Talk | contribs)
September 29 - October 5
goal: Isolate mrfp insert out of pSB1C3 and ligate in pIL253 and pNZ8048g to make biobrick compatible vectors for L. lactis
mrfp was primed out of pSB1C3 by primers designed for pIL253 and pNZ8048g
The reactions were loaded on a gel and this yielded positive results for the constructs for both pIL253 and pNZ8048g
The backbones pIL253 and pNZ8048g are digested so that the mrfp constructs will fit in them
Also the mrfp inserts are digested with the same or complementary enzymes
The backbones and mrfp inserts are checked on a gel
The gel did not yield good results for the pNZ mrfp
