Team:Evry/Interlab Study/09-16-2014

Construction n°1: PSB1C3 with I20260

Construction n°2: PSB1C3 with J23101-E1010

Construction n°3: PSB1C3 with K823012-E1010

Other constructions of the Anderson library of constitutive promoters

PSB1C3+E0240+J23100:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.

PSB1C3+E0240+J23101:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.

PSB1C3+E0240+J23102:

• glycerol stock preparation from LB culture from the 14th September.
• Measure of the cells' growth and fluorecence on TECAN infiniteM200.(18h30-1h30):

PSB1C3+E0240+J23106:

• glycerol stock preparation from LB culture from the 14th September.
• Measure of the cells' growth and fluorecence on TECAN infiniteM200.(2h-9h):

PSB1C3+E0240+J23115 - PSB1C3+E0240+J23115:

• PCR colony of 5 colonies for each construction following protocol table 1 and 2.
  

  

  GEL 1: 1% agarose gel. PCR product of 5 colonies of the PSB1C3+E0240+J23116 and PSB1C3+E0240+J23115 constructions.

A pre-culture was made with one colony for each construction to make a glycerol stock.

PSB1C3+E0240+J23117 - PSB1C3+E0240+J23119:

• PCR colony of 6 colonies for each construction following protocol table 1 and 2.
  

  

  GEL 2: 1% agarose gel. PCR product of 6 colonies of the PSB1C3+E0240+J23117 and PSB1C3+E0240+J23119 constructions.

A pre-culture was made with one colony for each construction to make a glycerol stock.


Sep 16