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Team:Nankai/Characterization
From 2014.igem.org
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What we have to do is insert the genes rhlABRI to the plasmid pBBR1MCS-2. To accomplice this, we figure out 4 steps as followed.
1.Restriction endonuclease site
Considering P. aeruginosa SQ6 is a wild-type strain, we retained the genes’ native promoter from the strain. Comparing the restriction sites of multiple cloning sites of the plasmid pBBR1MCS-2 and the genes rhlABRI including the promoter, we chose the restriction endonuclease sites, Hind III and Sal I.
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vel tempus libero diam vel arcu. Etiam id tincidunt tortor. Nam auctor consequat quam, vel mattis dui laoreet a. Nunc condimentum iaculis tortor, id eleifend nulla mattis lobortis. Pellentesque semper blandit odio, id tempor lorem imperdiet eu. Ut sagittis sagittis consectetur ,Maecenas eget risus eros. Nunc venenatis ante a rutrum cursus.
Commodo at blandit vitae, placerat in sem. Morbi ornare nec felis in euismod. Suspendisse vulputate orci ultrices enim facilisis, vel lobortis magna rhoncus. Integer mattis at elit vitae adipiscing. Cras imperdiet cursus nunc quis ullamcorper.
