From 2014.igem.org

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2^nd June

• DAY TWO : Inoculate 1L SOB with 12 mL overnight culture. Grow culture at 18°C (this temperature is really important as we see a 10-fold decrease in competency when we grow them at room temperature)
• Preparation of LB medium (Agar for plates, +Ampicillin and +Chloramphenicol)