Team:TCU Taiwan/Notebook

From 2014.igem.org

Revision as of 18:17, 17 October 2014 by House (Talk | contribs)

<script>

 (function(i,s,o,g,r,a,m){i['GoogleAnalyticsObject']=r;i[r]=i[r]||function(){
 (i[r].q=i[r].q||[]).push(arguments)},i[r].l=1*new Date();a=s.createElement(o),
 m=s.getElementsByTagName(o)[0];a.async=1;a.src=g;m.parentNode.insertBefore(a,m)
 })(window,document,'script','//www.google-analytics.com/analytics.js','ga');
 ga('create', 'UA-48805552-2', 'igem.org');
 ga('send', 'pageview');

</script>

<style type="text/css">

 #jamboreemenu ul {list-style: none;padding: 0px;margin: 0px;}
 #jamboreemenu ul li {display: block;position: relative;float: left; #A66B38; width:150px;}
   #jamboreemenu li ul {display: none;}
   #jamboreemenu ul li a {display: block;background: #A66B38;  width:150px; padding: 10px 5px 10px 10px;text-decoration: none;
          white-space: nowrap;color: #fff;}
 #jamboreemenu li a:hover {background: #F2DE79;  width:150px;}
   #jamboreemenu li:hover ul {display: block; position: absolute;}
  #jamboreemenu li:hover li {float: none;}
 #jamboreemenu  li:hover a {background: #F2DE79; width:150px;}
 #jamboreemenu  li:hover li a:hover {background: #A66B38; width:150px;}
 #drop-nav li ul li {border-top: 10px; }
 #gotop {
   display: none;
   position: fixed;
   right: 20px;
   bottom: 20px;    
   padding: 10px 15px;    
   font-size: 20px;
   background: #777;
   color: white;
   cursor: pointer;

} fieldset { margin-bottom:10px; border-color:#82C0AF; } </style> <script type="text/javascript">

$(function(){

   $("#gotop").click(function(){
       jQuery("html,body").animate({
           scrollTop:0
       },1000);
   });
   $(window).scroll(function() {
       if ( $(this).scrollTop() > 300){
           $('#gotop').fadeIn("fast");
       } else {
           $('#gotop').stop().fadeOut("fast");
       }
   });

}); </script>

<body style="background:#ffffff">

  <img src="TCU-Notebook-logo.png" width="128" height="128">
Notebook
<img src="TCU-Logo-up.png" width="90%">  
<tbody>
<tbody> </tbody>

<tr><td colspan="3"background="#F3E4C2"><img src="TCU-Line.png" width="100%"></td></tr>

</tbody></table>



iGEM materials
<fieldset>
 <legend class="qnum" align="center">Bacteria medium
</legend>

*LB(Luria-Bertani) broth: 1% Trytone, 1% NaCl, 0.5% Yeast extract in the ddH2O

  • LB agar plate:LB broth add 1.5% agar.If you want to add selection marker example for ampicillin or chloramphenicol. To culture E.coli, ampicillin is added 100 mg/ml for usage, chloramphenicol is added 30 mg/ml for usage, kanamycin is added 70 mg/ml for usage

   </fieldset>
<fieldset>
 <legend class="qnum" align="center">Plasmid isolation
</legend>

*Solution I: 50 mM glucose、25 mM Tris-HCl (pH 8.0)、10 mM EDTA

  • Solution II:0.2N NaOH and 1% SDS(Sodium dodecyl sulfate ) mix
  • Solution III: 5 M potassium acetate (pH4.8), 11.5% glacial acetic acid
  • RNase A(10 mg/ml)
  • Phenol chloroform
  • Chloroform
  • 95% Ethanol
  • Tris-EDTA(TE)buffer:10 mM Tris-Cl(pH 7.5) 1 mM EDTA
  • pBluescript SK(-)
  • BBa_K1218011
  • BBa_K914003
  • BBa_I13521

   </fieldset>
<fieldset>
 <legend class="qnum" align="center">Quick screening
</legend>

*Quick screening buffer(Tris-Cl pH 12.6, 3% SDS)

  • Phenol chloroform
  • Chloroform

   </fieldset>
<fieldset>
 <legend class="qnum" align="center">Enzyme digestion

</legend>

*T4 ligase

  • 10X ligation buffer
  • EcoRI
  • PstI
  • SpeI
  • XbaI
  • BSA 0.1%

   </fieldset>
<fieldset>
 <legend class="qnum" align="center">Bacteria strains and others
</legend>

*DH5a
*JM101
*0.8% and 2% agarose gel
*95% Ethanol
*6X loading dye
*1.5 ml centrifuge tube
*250 ml flask
*M13KO7(1*1011 pfu/ml)from NEW ENGLAND BioLabs

   </fieldset>
<fieldset>
 <legend class="qnum" align="center">Polymerase chain reaction
</legend>

*Top DNA polymerase

  • dNTP(dATP, dCTP, dGTP, dTTP) 250 mM
  • Tris-HCl(pH9.0) 10 mM
  • KCl 30 mM
  • MgCl2 1.5 mM

   </fieldset>
<fieldset>
 <legend class="qnum" align="center">Instruments
</legend>

*VCM-620, laminal flow

  • Biofuge pico, Heraeus
  • OS1500-H, TSK Oribital Shaking Iucubator
  • Biowave
  • Vortex-GENE 2
  • Autoclave AS-1060L
  • GenAmp PCR System 2400, Applied Biosystems
  • BU-420, YIHDER
  • 5804R, eppendorf Centrifuge

   </fieldset>
Life Records
   
 
<iframe src='http://cdn.knightlab.com/libs/timeline/latest/embed/index.html?source=0An3IHGTTAU59dFRRcGpFa0lucU92TzUxZ0hFYWZKaVE&font=Bevan-PotanoSans&maptype=toner&lang=en&height=650' width='100%' height='650' frameborder='0'></iframe>
 
 
Study Together
 
<iframe width="720" height="482" frameborder="0" src="http://files.slidemypics.com/app/js/iframe.html?bg_color=fafafa&hash=67635bbf913055c734b13d75a1461392&r=0.02183609106577933"></iframe>
 
 
Conference in NCTU
 
<iframe width="720" height="482" frameborder="0" src="http://files.slidemypics.com/app/js/iframe.html?bg_color=ffffff&hash=035d3355e0b6454be558e6d9a1461398&r=0.9810947910882533"></iframe>
 
Trip in Chihsingtan Beach
 
<iframe width="720" height="482" frameborder="0" src="http://files.slidemypics.com/app/js/iframe.html?bg_color=ffffff&hash=fb44d802e37470fe77b56b41a1461402&r=0.6266446833033115"></iframe>
 
 
 
<img src="TCU-Line.png" width="100%">
^
   <a href="https://2014.igem.org/Team:TCU_Taiwan/Contact" title="Contact Us"><img src="TCU-Contact-bot.png" width="80" onMouseMove="this.src=Image0.src;" onMouseOut="this.src='https://static.igem.org/mediawiki/2014/8/84/TCU-Contact-bot.png'"></a>
   

<a href="mailto: tcutaiwan@gmail.com" target="_blank" title="Gmail"><img src="TCU%E3%84%A6Gmail.png" width="30"></a>  <a href="https://www.facebook.com/TCUiGEM?ref=ts&fref=ts" target="_blank" title="Facebook"><img src="TCU-Facebook.png" width="30"></a> <a href="https://www.youtube.com/channel/UCr40ZmaFNJGx7tUufCXK_OQ" target="_blank" title="YouTube"><img src="TCU_Youtube.png" width="30"></a> <a href="http://tcuigem.wordpress.com/" target="_blank" title="Wordpress"><img src="TCU-Wordpress.png" width="30"></a>
Team Members Project Parts Human Pratics Modeling Safety Notebook Attributions
<a href="https://2014.igem.org/Team:TCU_Taiwan/Team" title="Team Members"><img src="TCU-Team-logo.png" width="100"onMouseOver="this.width=this.width*1.1;Members.color='#333'" onMouseOut="this.width=this.width/1.1;Members.color='#F2DE79'"></a> <a href="https://2014.igem.org/Team:TCU_Taiwan/Project" title="Project"><img src="TCU-Project-logo.png" width="100" onMouseOver="this.width=this.width*1.1;Project.color='#333'" onMouseOut="this.width=this.width/1.1;Project.color='#F2DE79'"></a> <a href="https://2014.igem.org/Team:TCU_Taiwan/Parts" title="Parts"><img src="TCU-Part-logo.png" width="100" onMouseOver="this.width=this.width*1.1;Parts.color='#333'" onMouseOut="this.width=this.width/1.1;Parts.color='#F2DE79'"></a> <a href="https://2014.igem.org/Team:TCU_Taiwan/HumanPratics" title="Human Pratics"><img src="TCU-Human-logo.png" width="100" onMouseOver="this.width=this.width*1.1;Pratics.color='#333'" onMouseOut="this.width=this.width/1.1;Pratics.color='#F2DE79'"></a> <a href="https://2014.igem.org/Team:TCU_Taiwan/Modeling" title="Modeling"><img src="TCU-Modeling-logo.png" width="100" onMouseOver="this.width=this.width*1.1;Modeling.color='#333'" onMouseOut="this.width=this.width/1.1;Modeling.color='#F2DE79'"></a> <a href="https://2014.igem.org/Team:TCU_Taiwan/Safety" title="Safety"><img src="TCU-Safety-logo.png" width="100" onMouseOver="this.width=this.width*1.1;Safety.color='#333'" onMouseOut="this.width=this.width/1.1;Safety.color='#F2DE79'"></a> <a href="https://2014.igem.org/Team:TCU_Taiwan/Notebook" title="Notebook"><img src="TCU-Notebook-logo.png" width="100" onMouseOver="this.width=this.width*1.1;Notebook.color='#333'" onMouseOut="this.width=this.width/1.1;Notebook.color='#F2DE79'"></a> <a href="https://2014.igem.org/Team:TCU_Taiwan/Attributions" title="Attributions"><img src="TCU-Attributions-logo.png" width="100" onMouseOver="this.width=this.width*1.1;Attributions.color='#333'" onMouseOut="this.width=this.width/1.1;Attributions.color='#F2DE79'"></a>

Lost the way? Use it to help you if you're lost.

<a href="https://2014.igem.org/Team:TCU_Taiwan" title="Home"><img src="TCU-Logo-d.png" width="100"></a>

<img src="http://i.creativecommons.org/l/by/3.0/88x31.png" width="100">

^

</html>