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Team:ZJU-China/Log
From 2014.igem.org
| Team Sponsor | Contact us | ||
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Team Forum(BBS): | ZJU-China 2014 Team Forum on www.zjubiolab.zju.edu.cn | |
| Post Address: | Biolab Center Room 413, ZJU Zijin'gang Campus, Yuhang Tang Road No.866, Hangzhou, Zhejiang |
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| Powered by Media Wiki. Zhejiang University. | Email Address: | zjuchina2014 @ 163.com | |
GENE SOCKET DATA AND STRATEGY!
GENE SOCKET uses a plasmid, a circuit and a strain to construct circuits on chromosome by lambda-red and ecombinase-derived bistable switch. BBa_K1433018, which is called SOCKET, is a circuit need to be recombined on chromosome by a helper plasmid BBa_K1433013, called SUPPORT DEVICE.!
TO achieve our project, we designed 8 exquisite circuits, 3 on plasmid and 5 on chromosome. See our Circuit Strategy.pdf!
TO construct this circuit, we use GBclonart Seamless Assembly Kit.
TO prepare segments which the kit required and to test circuit accuracy, we designed hundreds of primers. See our Primer.pdf!
TO use these segments which have prepared by using the primers through assembly kit , we did lots of PCR. See our PCR Strategy.pdf!
TO get some segments which cannot find in The 2014 DNA Distribution Kit, we designed 4 fragments and acquire them by de novo synthesis. See our Total Synthesis Strategy.pdf!
TO assembly these segments and construct our circuits by assembly kit, we did lots of work. See our Assembly Kit.pdf!
TO verify the accuracy of constructed circuits, we choose colony PCR. See our Colony PCR.pdf!
TO recombine these circuit on chromosome, we use lambda red homologous recombination in many different strains. See our lambda red.pdf and Strain.pdf.
TO deliver our circuit as parts, we use PCR, restriction enzymes or DNA ligases. See our Parts Strategy.pdf!
WHAT’S more, we helped team Peking construct a circuit and do some testing experiments. See our PKU primer.pdf!
WE have quite minute and clearly lab record every day during whole project period. See our Lab record demo.pdf and
