Team:Tuebingen/Notebook/Protocols/LB agar

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Protocols

LB agar

Reagents

10 g Bacto-tryptone
5 g yeast extract
10 g NaCl

 

Procedure

  1. Add the reagents to 800 ml H2O
  2. Adjust pH to 7.5 with NaOH
  3. Add 15g agar
  4. Melt agar into solution in the microwave
  5. Adjust volume to 1 L with dH2O
  6. Sterilize by autoclaving

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