Team:Tuebingen/Notebook/Protocols/LB agar

From 2014.igem.org

Revision as of 15:32, 17 October 2014 by SvenB (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Protocols

LB agar

Reagents

10 g	Bacto-tryptone
5 g	yeast extract
10 g	NaCl

Procedure

Add the regents to 800ml H₂O
Adjust pH to 7.5 with NaOH
Add 15g agar
Melt agar into solution in the microwave
Adjust volume to 1L with dH₂O
Sterilize by autoclaving

Retrieved from "http://2014.igem.org/Team:Tuebingen/Notebook/Protocols/LB_agar"