Microfluidics Platform

To optimize DNA synthesis using our De Novo Synthesis BioBrick, a microfluidics platform is being developed. It is unrealistic to have a lab worker pipetting for hours upon hours to produce large DNA sequences. Our system would greatly aid in the process by simplifying and automating the process.

This robotic arm is the first scaled down version of our automated system. This device was constructed in order to facilitate element timing using our in house TdT. The robotic arm, powered with a Printrboard, would lower the microfuge tube into the appropriate water baths for the experimental time periods. Peristalsis pumps made from common NEMA-17 stepper motors, would pump in and out the correct solutions in the any deesired sequence. Between cycles, the DNA would be held in the tube using an electromagnet and the solution would be rinsed in preparation for the next cycle. The Electromagnet would also allow indirect agitation of the DNA in the tube by rapidly switching the polarity of the electromagnet using a solid state relay. This device can deliver volumes as low as 10μl, making the robotic arm more an efficient "macrofluidic" device. Although it seems complicated, many of the parts are the same as those found in the typical 3D printer and can be easily sourced.

Macro Arm Cad

Parallelogram arm with turret base allows for two degrees of freedom: up/down and left/right.

Assembly

Fluidics Pump

Macro Pump Cad

Universal NEMA-17 peristalsis pump. It uses 2mm silicon tubing and four 8mm bearings. Everything else is 3D printed.

Micro Sketch

In order to minimize the consumption of our in house TdT and other reagents, a microfluidic device would be a more ideal platform for automation. The microfluidics platform has not yet been completed and is still in progress. We are hoping to prototype out a 10-50x scale version from a 3D print before pursuing soft lithography.