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Team:Groningen/Template/MODULE/notebook
From 2014.igem.org
Notebook
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Appendix
Primers for making BioBricks out of the nisin operon
| Primer | Sequence |
|---|---|
| NisA forward | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGTACAAAAGATTTTAA |
| NisA reverse | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATTTGCTTACGTGAA |
| NisB forward | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGATAAAAAGTTCATTTAA |
| NisB reverse | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATCATTTCATGTATTCTT |
| NisT forward | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGATGAAGTGAAAGAATTTACATCA |
| NisT reverse | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATTCATCATTATCCT |
| NisC forward | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAATAAAAAAAATATAAA |
| NisC reverse | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATCATTTCCTCTTCCCTC |
| NisI forward | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGAAGATATTTAATACT |
| NisI reverse | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTACTAATTTCCTACCTTCG |
| NisP forward | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAAAAAAATACTAGGTTT |
| NisP reverse | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATCAATTTTTAGTCTTTC |
| NisRK forward | GTTTCTTCGAATTCGCGGCCGCTTCTAGGTGTATAAAATTTTAATAGT |
| NisRK reverse | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTACTTTTTTATTTTTA |
| NisFEG forward | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCAGGTAAAAATTCAAAA |
| NisFEG reverse | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATCTAATCTTTTTTT |
| PNisI+RBS forward | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGCACTGGATAATGACTATT |
| PNisI+RBS reverse | TACTAGTAGCGGCCGCTGCAGGAAGAAACTTCCTCTTCCCTCCTTTCAA |
Primers for making the new sfGFP(Bs) BioBrick
| Primer | Sequence |
|---|---|
| sfGFP(Bs) forward | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTCAAAAGGAGAAGAGCT |
| sfGFP(Bs) reverse | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTACTTATAAAGCTCAT |
Primers for making BioBricks out of genes with illegal restriction sites
The primers are denoted with the position of the
illegal restriction site that they should remove. Illegal EcoRI sites
are abbreviated with an E, XbaI sites with an X, SpeI site with an S and
PstI sites with a P.
| Gene | Primer | Sequence |
|---|---|---|
| NisT | Prefix forward | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGATGAAGTGAAAGAATTTACATCA |
| S980 reverse | TTGTTCCATAAACAAACTTGTATTATAAATGATGTAAATA | |
| S980 forward | TATTTACATCATTTATAATACAAGTTTGTTTATGGAACAA | |
| S1210 reverse | CCATAGTTGGTTGATATAATCCTGAAATTATCTTTACTTGTGTAC | |
| S1210 forward | GTACACAAGTAAAGATAATTTCAGGATTATATCAACCAACTATGG | |
| Suffix reverse | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATTCATCATTATCCT | |
| Suffix forward | ATGAATAATAATACTAGTAGCGGCCGCTGCAGGAAGAAACAAAAGGGCAA | |
| Prefix reverse | CACTTCATCCATCTAGAAGCGGCCGCGAATTCGAAGAAACATCCTTAGCG | |
| NisP | Prefix forward | GTTTCTTCGAATTCGCGGCCGCTTCTAGGTGAAAAAAATACTAGGTTT |
| P225 reverse | AGTCCTTGTTGTCGATTTTACTGCTGGTGACTGCGCCCCT | |
| P225 forward | AGGGGCGCAGTCACCAGCAGTAAAATCGACAACAAGGACT | |
| E347 reverse | AACGCTATCTCTCTTACTAAATTCAGAACTAACTTGAGTT | |
| E347 forward | AACTCAAGTTAGTTCTGAATTTAGTAAGAGAGATAGCGTT | |
| E1657 reverse | CAATACTCTATTCCCATTAACTTCTGGACTATTCATTAGA | |
| E1657 forward | TCTAATGAATAGTCCAGAAGTTAATGGGAATAGAGTATTG | |
| Suffix reverse | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATCAATTTTTAGTCTTTC | |
| Suffix forward | AAATTGATAATACTAGTAGCGGCCGCTGCAGGAAGAAACAAAAGGGCAA | |
| Prefix reverse | CCTAGTATTTTTTTCATCTAGAAGCGGCCGCGAATTCGAAGAAACATCCTTAGCG | |
| NisFEG | Prefix forward | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCAGGTAAAAATTCAAAA |
| P186 reverse | TCCAGTATCAGCAGAAATTAAACCAAACAAAATTTTCATC | |
| P186 forward | GATGAAAATTTTGTTTGGTTTAATTTCTGCTGATACTGGA | |
| E336/X349 reverse | CCTGTTTCTGCCAAACCAATCACTTCTAGTGTTTCATGTATTCTCTTA | |
| E336/X349 forward | TAAGAGAATACATGAAACACTAGAAGTGATTGGTTTGGCAGAAACAGG | |
| Suffix reverse | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATCTAATCTTTTTTT | |
| Suffix forward | TAGATAATAATACTAGTAGCGGCCGCTGCAGGAAGAAACAAAAGGGCAA | |
| Prefix reverse | TTTTACCTGCATCTAGAAGCGGCCGCGAATTCGAAGAAACATCCTTAGCG |
