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From 2014.igem.org

BYU 2014 Notebook Edit July August

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7/07/2014 - Garrett Jensen.
- Today I looked around for the M17 media that supposedly was delivered 2 weeks ago. Brother Lee and Dr. Robison both said that it was not in their lab but they are the only ones who signed out a package from Fischer Scientific 2 weeks ago.

7/09/2014 - Garrett Jensen.
- Today I ran a PCR on the DNA that Skip isolated from S. thermophilus on June 26 using the universal 16S ribosomal primers. I used taq polymerase instead of Redtaq because the redtaq has been having some issues lately. If there is microbial DNA present then we may be able to get PCR for the CRISPR to work from it. In the meantime I am waiting for our M17 media to show up.

7/10/2014 - Garrett Jensen.
- I ran out that PCR on a gel and there was a Band!!!! That means that there is good DNA present. I started a PCR reaction using taq polymerase and our Crispr primers to see if I can get a product for the CRISPR system. If I can then I will set it up again with phusion. We still have not received the m17 media however.

7/16/2014 - Garrett Jensen.
- On monday (7/14/2014) we moved our lab to the new life sciences building and were not able to do any work.
- I ran out the PCR attempt to amplify the CRISPR from the LMD-9 DNA that Skip made but no bands were visible.
- After talking with Dr. Grose and Desi we decided to cancel our order of the M17 media. It apparently was back ordered but the company sent a shipping document anyways. We have all the materials to make M17 media except for beef extract. Desi found a place to order it from for pretty cheap and sent the information to Dr. Grose. We were able to find ascorbic acid (vitamin C) in Dr. O'Neil's lab. Once the beef extract comes in I can grow LMD-9 and purify its DNA.