Results
GFP＋HlyA(BBa_K1414000) and HlyA＋GFP(BBA_K1414001)
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- [Figure 1]
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GFP
We used "BBa_E0040".
Length of "BBa_E0040"is 920bp.
Following figure 1 is result of the GFP PCR.
As a result,We were able to confirm a band about 920bp by GFP PCR.
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- [Figure 2]
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HlyA
We used "BBa_K554002".
Length of "BBa_K554002" is 186bp.
Following figure 2 is result of the HlyA PCR.
As a result,We were able to confirm a band about 186bp by HlyA PCR.
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- [Figure 3]
- [Figure 4]
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GFP＋HlyA and HlyA＋GFP
GFP ligated with HlyA.Length of GFP＋HlyA is 920bp.
Following figure 3 is GFP＋HlyA.
Following figure 4 is HlyA＋GFP.
We submitted these parts.
But the function of these parts,we were not able to confirm it.
Because we did not have time to experiment.
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Creating parts of STAT3
STAT3's sequence includes three recognition sequences of restriction enzyme.
We try to mutant this sequence,and we could succeed to mutant about one place.
Creating parts of IL-10α,IL-10β
IL-10a's sequence includes six,IL-10b's sequence includes three recognition sequences of restriction enzyme.
We try to mutant these sequences ,but we didn't succeed.
Creating parts of HRV,IL-5Ra,IL-5Rb,AraC,Arac Promoter
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- [Figure 1]
- [Figure 2]
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Result of colony PCR
Figure 1 is colony PCR of colony transformed TA vector.
Lane 1,2 are colony transformed TA vector inserted AraC gene.
Lane 3,4 are colony transformed TA vector inserted AraC promoter gene.
Lane 5,6,7 are colony transformed TA vector inserted HRV gene.
The band of lane 5 was 325bp.
Objective band size was 325bp.
We are able to confirm that we get colony which has TA vecter inserted AraC promoter gene.
Figure 2 is colony PCR of colony transformed TA vector inserted AraC gene.
The band of lane 1,2 were 1104bp.
Objective band size was 1149bp.
We are able to confirm that we get colony which has TA vector inserted AraC gene.
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