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From 2014.igem.org

Contents 1 Wednesday 9th July 1.1 Lab work 1.1.1 1 - Plasmid DNA Extraction: 1.1.2 2 - Electrophoresis: 1.1.3 3 - New culture: 1.2 Reunion

Wednesday 9th July

Lab work

1 - Plasmid DNA Extraction:

Romain & Sean

Plasmids used: DH5αZ1 P5B1C3 RBS amilCP Ter clone 9, A and B.

Protocol

2 - Electrophoresis:

Arnaud & Romain & Sean

Plasmids used:

• DH5αZ1 P5B1C3 RBS amilCP Ter clone 9, A.
• DH5αZ1 P5B1C3 RBS amilCP Ter clone 9, B.
• XL1-Blue pCola-ges Kan
• pJBEI-6409 Cm

Protocol

3 - New culture:

Romain

New liquid cultures of:

• FNR-RBS-amilCp-Ter DH5αZ1 P5B1C3 Cm
• BBa B0015 dble ter P5B1C3 Cm

4x 5ml LB + 20μl Chloramphenicol + a little of bacteria. We incubate cultures at 37°C.

Reunion

Conference with the team to discuss about the project. The handled topics were:

• Definition of Biobricks to make
• Planning of internships
• Definition of Biobricks to render
• Understanding the construction of consecutive genes
• Organisation and planning of experiences to do
• Sponsoring
• Wiki's progress
• Modelling's progress

People there:

• Instructors and advisors: Alice, Olivier, Solenne and Sylvie.
• Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.

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