"
Team:Paris Saclay/Notebook/July/3
From 2014.igem.org
Revision as of 13:45, 11 July 2014 by Romain.sanson (Talk | contribs)
Thursday 3rd July
Lab Work
1 - Phages P1 transduction in MG1655Z1
Mathieu
In 2ml Eppendorf :
- 4 eppendorf MG1655 (0.1ml + 50µl CaCl)
- 4 eppendorf MG1655Z1 (0.1ml + 50µl CaCl)
Add 100µl of the different phages. Wait 20 minutes!
Add in each eppendorf :
- 1ml LB liquid
- 500µl citrate at 5.10^-3M
Wait 1 or 2 hours at 37°C with agitation
