Team:Groningen/Template/MODULE/Notebook/secretion/week3

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6 - 8 of August
 
Goal: obtain all the biobricks necessary for the secreting systems of P. aeruginosa and S. aureus.
 
E. coli was chemically transformed with 3 biobricks  in order to obtain them:
 
BBa_I746104: P2-promotor
BBa_K081009: LasI (for the Detection part)
BBa_C0060: Aiia
 
 
After which 1, 10, 20, 50% was inoculated on Chloramphenicol LB-agar plates.