Team:Northwestern/CFPS

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Cell-Free Protein Synthesis

20140723 Testing our lysates
  • data from BL21 (E.Coli) lysates
  • out of K12, DH5a, and BL21, BL21 expressed the most fluorescence
  • the plasmid used was superfolder GFP from Jewett Lab
20140915 All of our lysates
  • RBS B0034
  • Tried all of the E.Coli lysates as well as the environmental ones
  • spinach aptamer--> GFP