Risk assessment of the E. coli bacteria modified with genes of fluorescence

• Introduction
• Methodology
• Biosafety
• Achievements
• Bibliography

Biosafety

Good Security Practices (GSP) were taken out of a publication made by the Worldwide Health Organization (WHO) titled “Biosecurity manual in the lab” in 2005, and adapted it to the practice performed. This guaranteed the safety of the staff members that worked in the modification and testing of the bacterium. It is important to mention that the organism does not represent a biologic risk because of it been non-pathogenic. None the less, for the development of this project it was necessary to operate under a biosafety level 2 with the intention of learning how to apply better GSPs. In order to achieve this objective, the practice was based in 4 fundamental axes of biosafety:

• Restricted access for people not authorized

• Personal protection equipment team manages the instruments in the lab.

• Biocontention mechanisms.

• Final disposal methods.

Thanks to the GSP and other basic measures of biosafety the happening probability estimation and the consequence of this to happen be meaningless.