The particular aim of the second module is to realize the carbon dioxide fiaxtion in . For this approach The second module aims to change E. coli in a way that it binds carbon dioxide. This changes this bacterium from heterotroph to autotroph. To achieve this we want to establish the Calvin-cycle. E. coli has all enzymes for the Calvin-cycle except of three.
Figure1: Missing enzymes in Calvin cycle
The different results for all three enzymes are mentioned in the Calvin-cycle section. One important step for the carbon dioxide fixation is the RuBisCO (Ribulose 1,5-bisphosphate carboxylase/oxygenase). We decided to transform DNA sequences into E. coli which encode the carboxysome. Due to its special properties this microcompartiment is very usefull for the carbon dioxide fixation.
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