Bio Bricks

BBa_K1470000: mCat-1

mCat-1 is the receptor we introduced to human cells to be able to infect them using the MuLV

BBa_K1470001: Puromycin N-acetyl-transferase

Puromycin N-acetyl-transferase is an enzyme which we used for selecting stable eucaryotic cell lines. Because of the blocked protein biosynthesis more than 99% of uninfected cells will be dead within two days.

BBa_K1470002: Galactose-induced gene 4 (Gal4BD)

Galactose-induced gene 4 (Gal4BD) is used to investigate gene expression levels.

BBa_K1470003: Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element (WPRE)

Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element (WPRE) is a regulatory RNA sequence, which promotes viral gene expression.

BBa_K1470004: Secreted Alkaline Phosphatase (SEAP)

Secreted Alkaline Phosphatase (SEAP) is used in cell culture assays to quantify gene expression.

BBa_K1470005: engineered PDZ domain (ePDZ)

engineered PDZ domain (ePDZ) is an important part of the blue light system.

BBa_K1470006: The Viral Vector (pMIG)

The Viral Vector (pMIG) is the plasmid which is used to tranfect Phoenix cells with the gene to be packed into viral particles. It contains two LTRs and a multiple cloning site.

BBa_K1470007: Cas9-Nickase

Cas9-Nickaseis a Cas9 without iGEM restrction-sites but full catalytic activity.

Plasmids

mCat-1 Plasmids

Constitutive Expression

p14rz_003: pcDNA_SLC7A1_GFP

- bla

- blaII

p14rz_004: pcDNA_SLC7A1_HA

- bla

- blaII

p14rz_005: pcDNA_SLC7A1_mCherry

- bla

- blaII

p14rz_006: pcDNA_SLC7A1_HA_p2a_mCherry

- bla

- blaII

p14rz_007: pcDNA_SLC7A1_HA_mCherry

- bla

- blaII

Red Light induced

p14rz_010: TetO13_CMVmin_SLC7A1_HA_p2a_mCherry

- bla

- blaII

p14rz_011: TetO13_CMVmin_SLC7A1_HA_mCherry

- bla

- blaII

Blue Light induced

p14rz_008: Gal4_SLC7A1

- bla

- blaII

p14rz_009: Gal4_SLC7A1_HA

- bla

- blaII

Light System

Red Light

Blue Light

UV Light

p14ls_001: COP1-VP16_IRES_TetR-UVR8

- bla

- blaII

p14ls_002: Gal4_mCherry

- bla

- blaII