Team:Groningen/Template/MODULE/projects/parts/detection

(BBa_K1365997 , BBa_K1365998 & BBa_K1365999)

To give our LactoAid the ability to detect Pseudonomas aeruginosa we assembled a new biobrick. For the detection we adopted a widely used principle for detection, namely detection of quorum molecules. P. aeruginosa produces AHLs for communication [Source] and we build an AHL sensor (BBa_K1365997) designed for L. lactis.

To test the how well our LactoAid detects Pseudonomas aeruginosawe build a construct which has the AHL senor ability coupled with an super fold GFP (BBa_K1365998) .

The third part made for the detection is an AHL generator (BBa_K1365999). Many iGEM teams are working with AHL sensing, either to detected Pseudonomas aeruginosaor for an regulatory mechanism. Our AHL generator produces AHLs which enables other teams to produce AHLs and test their sensors without the hassle of working with the pathogenic bacterium itself.