Purpose

The difference in the biology of plants unveils a need for new standards. We decided to write a Request for Comments (RFC) to unite the plant SynBio community by defining a common language for plant synthetic biology, extensible to all other Eukaryotes. Its syntax is based on the Golden Gate System1 cloning method which uses TypeIIS restriction enzymes; variations, such as GB2.02 and MoClo3, are already being used across the field. This RFC brings together their common features and sets a consensus across the plant field for construct assembly and part repositories.

Marchantia polymorpha, a primitive liverwort, is being established as a new model organism for plant Synthetic Biologyi4; Contrary to historical model species, the choice of M. polymorpha has preceded its establishment. This is an opportunity to set standards in a coherent and united way paving the way for faster and more efficient sharing of information and parts.

The fewer the rules defining a standard, the more widely that standard is adopted. The introduction of this new chassis with its novel tool kit is the occasion to allow more flexibility in the Registry5, opening it to wider usage and contributions.

Thank you to the UEA and Valencia iGEM teams for their collaborations.

Rise of the Titles

Third part here