"
Team:SUSTC-Shenzhen/Notebook/timeline.json
From 2014.igem.org
{
"timeline": {
"headline": "Lab Milestones",
"type": "default",
"text": "The steps to success.
", "asset": {
"media": "
",
"credit": "iGEM SUSTC-Shenzhen",
"caption": "Lab Milestones"
},
"date": [
{
"startDate": "2014,7,27",
"endDate": "2014,7,28",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Fundamental/TransEfficiency.htm'>Effiency Test</a>",
"text": "DH5α&BL21
", "tag": "Fundamental",
"classname": "",
"asset": {
"media": "
",
"thumbnail": "",
"credit": "ProfFan",
"caption": "Figure 1"
}
},
{
"startDate": "2014,8,17",
"endDate": "2014,8,22",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/Determining-the-appropriate-concentration-of-puromycin-for-cell-selection'>Determining the appropriate concentration of puromycin for cell selection</a>",
"text": "Prepare for selection
", "tag": "HeLa Cell",
"classname": "",
"asset": {
"media": "
",
"thumbnail": "",
"credit": "Lidan Zeng",
"caption": "Our cells!"
}
},
{
"startDate": "2014,7,18",
"endDate": "2014,7,18",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Plasmid-amplification-II-for-plasmid-pX330-EGFP'>Plasmid amplification II for pX330 & EGFP & verifications by electrophoresis</a>",
"text": "Prepare for selection
", "tag": "CRISPR",
"classname": "",
"asset": {
"media": "
",
"thumbnail": "",
"credit": "Mengqi Xu, Sijia Liu",
"caption": "Electrophoresis Snapshot"
}
},
{
"startDate": "2014,8,9",
"endDate": "2014,8,10",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Enzyme-digestion-agrose-gel-DNA-Extraction-for-PX330-vector'>Enzyme digestion & agrose gel DNA Extraction for PX330 vector</a>",
"text": "To prepare PX330 vector for the following ligation experiments
", "tag": "CRISPR",
"classname": "",
"asset": {
"media": "",
"thumbnail": "",
"credit": "Mengqi Xu, Sijia Liu",
"caption": "Eluted DNA"
}
},
{
"startDate": "2014,8,6",
"endDate": "2014,8,6",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/5*UAS-plasmids-Fill-in'>5*UAS plasmids Fill-in</a>",
"text": "To eliminate the EcoRI site on the 5*UAS plasmid
", "tag": "CRISPR",
"classname": "",
"asset": {
"media": "
",
"thumbnail": "",
"credit": "Mengqi Xu, Sijia Liu",
"caption": "Eluted DNA"
}
},
{
"startDate": "2014,8,10",
"endDate": "2014,8,10",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/PCR-for-mCherry'>PCR for mCherry</a>",
"text": "To get our target fragment\nof mCherry by PCR
", "tag": "CRISPR",
"classname": "",
"asset": {
"media": "",
"thumbnail": "",
"credit": "Mengqi Xu, Sijia Liu",
"caption": "Eluted DNA"
}
},
{
"startDate": "2014,8,9",
"endDate": "2014,8,10",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/UAS-Fill-in-second-time'>UAS Fill-in (The second\ntime)</a>",
"text": "The same as the first Fill-in experiment
", "tag": "CRISPR",
"classname": "",
"asset": {
"media": "
",
"thumbnail": "",
"credit": "Mengqi Xu, Sijia Liu",
"caption": "Eluted DNA"
}
},
{
"startDate": "2014,8,11",
"endDate": "2014,8,11",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Struggles-for-our-second-time-Fill-in'>Struggles for our second-time Fill-in</a>\n",
"text": "A real challenge, indeed.
", "tag": "CRISPR",
"classname": "",
"asset": {
"media": "",
"thumbnail": "",
"credit": "Mengqi Xu, Sijia Liu",
"caption": "Eluted DNA"
}
},
{
"startDate": "2014,8,11",
"endDate": "2014,8,11",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Verification-for-plasmid-PX330-MCherry-GAL4-PX330-MCherry-amplified-2014-8-9-by-restriction-digestion'>Verification for plasmid PX330-MCherry-GAL4,PX330-MCherry (amplified on 2014.8.9) by restriction digestion</a>",
"text": "Failure, as it is.
", "tag": "CRISPR",
"classname": "",
"asset": {
"media": "",
"thumbnail": "",
"credit": "Mengqi Xu, Sijia Liu",
"caption": "Eluted DNA"
}
},
{
"startDate": "2014,8,12",
"endDate": "2014,8,12",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Follow-up-for-verification-of-plasmid-PX330-MCherry-GAL4'>Follow-up for verification of plasmid PX330-MCherry-GAL4 and The results of 'Struggles for fill-in(the second time)'</a>",
"text": "Life full of cycles, but each iteration with happiness.
", "tag": "CRISPR",
"classname": "",
"asset": {
"media": "",
"thumbnail": "",
"credit": "Mengqi Xu, Sijia Liu",
"caption": "Eluted DNA"
}
},
{
"startDate": "2014,8,12",
"endDate": "2014,8,12",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Follow-up-for-Struggles-for-fill-in-the-second-time'>Follow-up for 'struggles for our second-time Fill-in'</a>",
"text": "Failure, again.
", "tag": "CRISPR",
"classname": "",
"asset": {
"media": "",
"thumbnail": "",
"credit": "Mengqi Xu, Sijia Liu",
"caption": "Eluted DNA"
}
},
{
"startDate": "2014,8,13",
"endDate": "2014,8,16",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/UAS-Fill-in-The-third-time'>UAS Fill-in (The-third-time)</a>",
"text": "Then try for another time.
", "tag": "CRISPR",
"classname": "",
"asset": {
"media": "",
"thumbnail": "",
"credit": "Mengqi Xu, Sijia Liu",
"caption": "Eluted DNA"
}
},
{
"startDate": "2014,8,14",
"endDate": "2014,8,18",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Verifications-for-struggles-for-our-second-time-Fill-in'>Verifications for 'struggles for our second-time Fill-in'</a>",
"text": "Results verified.
", "tag": "CRISPR",
"classname": "",
"asset": {
"media": "",
"thumbnail": "",
"credit": "Mengqi Xu, Sijia Liu",
"caption": "Eluted DNA"
}
},
{
"startDate": "2014,8,14",
"endDate": "2014,8,14",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Verifications-for-struggles-for-our-second-time-Fill-in'>Verifications for 'struggles for our second-time Fill-in'</a>",
"text": "Results verified.
", "tag": "CRISPR",
"classname": "",
"asset": {
"media": "",
"thumbnail": "",
"credit": "Mengqi Xu, Sijia Liu",
"caption": "Eluted DNA"
}
},
{
"startDate": "2014,8,18",
"endDate": "2014,8,20",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Successful-fill-in-plasmid-(G56-1)-amplification'>Successful fill-in plasmid (G56-1) amplification</a>",
"text": "Days of success.
", "tag": "CRISPR",
"classname": "",
"asset": {
"media": "",
"thumbnail": "",
"credit": "Mengqi Xu, Sijia Liu",
"caption": "Eluted DNA"
}
},
{
"startDate": "2014,8,20",
"endDate": "2014,8,20",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Point-mutation-PCR-for-UAS'>Point-mutation PCR for UAS</a>",
"text": "One approach.
", "tag": "CRISPR",
"classname": "",
"asset": {
"media": "",
"thumbnail": "",
"credit": "Mengqi Xu, Sijia Liu",
"caption": "Eluted DNA"
}
},
{
"startDate": "2014,8,21",
"endDate": "2014,8,21",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Point-mutation-PCR-for-mCherry-with-BbsI-site-mutation'>Point-mutation PCR for mCherry (with BbsI site mutation)</a>",
"text": "To eliminate the BbsI site on mCherry fragment.
", "tag": "CRISPR",
"classname": "",
"asset": {
"media": "",
"thumbnail": "",
"credit": "Mengqi Xu, Sijia Liu",
"caption": "Eluted DNA"
}
},
{
"startDate": "2014,8,21",
"endDate": "2014,8,22",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Endotoxin-Free-plasmid-purification-for-pX330-mCherry-pX330-mCherry-GAL4'>Endotoxin Free plasmid purification for pX330+mCherry, pX330+mCherry+GAL4</a>",
"text": "To prepare for further cell culturing.
", "tag": "CRISPR",
"classname": "",
"asset": {
"media": "",
"thumbnail": "",
"credit": "Mengqi Xu, Sijia Liu",
"caption": "Eluted DNA"
}
},
{
"startDate": "2014,8,22",
"endDate": "2014,8,22",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Verification-for-pX330-mCherry-pX330-mCherry-GAL4-enzyme-digestion'>Verification for pX330+mCherry, pX330+mCherry+GAL4 by enzyme digestion</a>",
"text": "To ensure that we have not made errors.
", "tag": "CRISPR",
"classname": "",
"asset": {
"media": "",
"thumbnail": "",
"credit": "Mengqi Xu, Sijia Liu",
"caption": "Eluted DNA"
}
},
{
"startDate": "2014,8,25",
"endDate": "2014,8,26",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Construction-5X-UAS-mCherry-pX330-7X-UAS-mCherry-pX330-I'>Construction of 5X-UAS-mCherry-pX330 & 7X-UAS-mCherry-pX330 I</a>",
"text": "",
"tag": "CRISPR",
"classname": "",
"asset": {
"media": "",
"thumbnail": "",
"credit": "Mengqi Xu, Sijia Liu",
"caption": "Eluted DNA"
}
},
{
"startDate": "2014,7,17",
"endDate": "2014,7,19",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Plasmid-amplification-I-for-plasmid-pX330-EGFP'>Plasmid amplification I for plasmid pX330 & EGFP</a>",
"text": "Prepare the necessary materials
", "tag": "CRISPR",
"classname": "",
"asset": {}
},
{
"startDate": "2014,9,29",
"endDate": "2014,10,07",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/Investigation-Cas9-protein-affect-cell-growth'>Investigation Cas9 Protein affect cell growth</a>",
"text": "DH5α&BL21
", "tag": "HeLa Cell",
"classname": "",
"asset": {
"media": "",
"thumbnail": "",
"credit": "ProfFan",
"caption": "Figure 1"
}
},
{
"startDate": "2014,8,04",
"endDate": "2014,8,05",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/Thawing-HeLa-Cell'>Thawing HeLa Cell</a>",
"text": "DH5α&BL21
", "tag": "HeLa Cell",
"classname": "",
"asset": {
"media": "",
"thumbnail": "",
"credit": "ProfFan",
"caption": "Figure 1"
}
},
{
"startDate": "2014,8,14",
"endDate": "2014,9,20",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/Construct-Cellline'>Constructing Cell Line</a>",
"text": "DH5α&BL21
", "tag": "HeLa Cell",
"classname": "",
"asset": {
"media": "",
"thumbnail": "",
"credit": "ProfFan",
"caption": "Figure 1"
}
},
{
"startDate": "2014,7,27",
"endDate": "2014,8,10",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/PB5-TetOn-Cas9-PB3-plasmid-construction'>PB5-TetOn-Cas9-PB3 Plasmid Construction</a>",
"text": "DH5α&BL21
", "tag": "HeLa Cell",
"classname": "",
"asset": {
"media": "",
"thumbnail": "",
"credit": "ProfFan",
"caption": "Figure 1"
}
},
{
"startDate": "2014,9,13",
"endDate": "2014,9,21",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/G1_cells_transfected_with_px330'>Transfect Cells with pX330</a>",
"text": "DH5α&BL21
", "tag": "HeLa Cell",
"classname": "",
"asset": {
"media": "",
"thumbnail": "",
"credit": "ProfFan",
"caption": "Figure 1"
}
},
{
"startDate": "2014,9,27",
"endDate": "2014,10,06",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/Determining-the-higher-concentration-of-balsticidin-for-cell-selection'>Determining the Increased Concentration of Balsticidin for Cell Selection</a>",
"text": "DH5α&BL21
", "tag": "HeLa Cell",
"classname": "",
"asset": {
"media": "",
"thumbnail": "",
"credit": "ProfFan",
"caption": "Figure 1"
}
}
],
"era": [
{
"startDate": "2014,7,27",
"endDate": "2014,8.7",
"headline": "Basic Research",
"text": "The Fundamentals
", "tag": "Fundamental"
}
]
}
}
