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Team:Paris Bettencourt/Project/Foot Odor
From 2014.igem.org
Toxic
| BACKGROUND Foot odor is generally perceived as socially awkward and negative.Bacillus subtilis, a common bacterium that lives on feet is one of the main responsible of the bad foot odor.One of the molecules produced by B. subtilis uses the leucine degradation pathway to produce isovaleric acid, which smells like parmesan cheese. |
AIMS There are a many commercially available solutions for this problem, current products indiscriminately target bacteria on the foot skin microbiome which can have negative effects on skin health and microbiome dynamics. We aim to develop a targeted approach to prevent foot odor, by selectively killing microbes responsible for the biosynthesis of volatile compounds. |
RESULTS Lorem ipsum dolor sit amet, consectetur adipiscing elit. Cras elementum mauris interdum lacus venenatis aliquet. In ac nulla semper, convallis orci non, vestibulum nisi. Maecenas quis ultricies tellus. |
| Aims and Achievement | Introduction | Results | Methods | References |
Aims and Achievement
Foot odor is generally perceived as socially awkward and negative. Although there are a many commercially available solutions for this problem, current products indiscriminately target bacteria on the foot skin microbiome. These type of products can have negative effects on skin health and microbiome dynamics. We aim to develop a targeted approach to prevent foot odor, by selectively killing microbes responsible for the biosynthesis of volatile compounds which compose the characteristic stinky feet smell, without destroying the beneficial microbes.
Introduction
Foot odor (or bromhidrosis) is a condition in which a particular type of body odor, generally considered to be unpleasant, gets produced by bacteria during the catabolism of nutrients present in the sweat. Although sweat is almost odorless, the microbial volatile compounds that are formed as by-products of bacterial metabolism are perceived as an unpleasant smell. Bacillus subtilis, a common bacterium that lives on feet and it is thought to produce the malodorous molecules behind this socially awkward condition. One of the molecules produced by B. subtilis uses the leucine degradation pathway to produce isovaleric acid, which smells like parmesan cheese. It is considered to be to dominant odor tone of bromhidrosis.
Fig2
Fig3
Results
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Fig1
Methods
We hypothesized that bad foot odor could be eradicated by perturbing the leucine degradation pathway. We chose leucine dehydrogenase and iso-valeryl coA to perturb the leucine degradation pathway. Our goal here is to have two different strategies to inhibit isovaleric acid production. our first strategy focus on producing B.subtilis which will not be capable of producing leucine, so that it has to utilize all the leucine it could get from the sweat for its own survival rather than the production of isovaleric acid. Our second strategy focus on developing B.subtilis strain, which will be capable of producing leucine but not isovaleric acid.
References
- ref1- ref2
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