Team:Cooper Union/Notebook/Biohack April

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Cooper Union 2014 iGEM

Biohacker Kit



3/13/14
Digested pBR322 with HindIII.
Double digested pBR322 with HindIII and EcoRI.

3/27/14
Ran digests from 3/13/14 on gel. 
Excised gel fragments with plasmid in them.
Purified gel fragments.

4/10/14
Phosphatase treated the digested and double digested pBR322 from 3/13/14.

4/17/14
Digested UV, HSP, and GFP g-blocks with HindIII and EcoRI. 
Performed five ligations:
	pBR322 with UV
	pBR322 with HSP
	pBR322 with GFP
	UV with GFP
	HSP with GFP

4/24/14
Purified UV+GFP and HSP+GFP ligations.
Digested the two ligations with HindIII.
Ligated each one into pBR322.