Team Example's Project name!

Project Introduction

CO₂ emissions have recieved a lot of attention in modern times, due to concerns that high emission levels are facilitating global warming. Consequently, a lot of research is focused on ways of reducing CO₂ emissions from industry, and ways of fixating atmospheric CO₂ at a greater than normal rate.

Our project is attempting to produce a BioBrick, which when placed inside photosynthetic bacteria, increases their rate of CO₂ fixation. In order to achieve this, we first need to construct a BioBrick that allows inducible expression of non native genes in our chassis; Synechocystis sp. PCC 6803

Results

• Result 1 - Lorem ipsum ad his scripta blandit partiendo, eum fastidii accumsan euripidis in, eum liber hendrerit an.
• Result 2 - Lorem ipsum ad his scripta blandit partiendo, eum fastidii accumsan euripidis in, eum liber hendrerit an.
• Result 3 - Lorem ipsum ad his scripta blandit partiendo, eum fastidii accumsan euripidis in, eum liber hendrerit an.

Reporter BioBrick

The first stage of this project consists of creating a reporter BioBrick in order to confirm that we have are able to induce the expression of foreign genes in Synechocystis sp. PCC 6803. This reporter BioBrick makes use of several other BioBricks, such as BBa_J23101, BBa_B0034, BBa_C0012 and BBa_E1010 .

Once the reporter plasmid has been successfully constructed, we will verify that it is working as intended by inducing the expression of a fluorescent protein contained on the plasmid. Once induced, the colonies of cells transformed with this plasmid should exhibit a color that is determined by the fluorescent protein.

The plasmid is transformed into Synechocystis sp. PCC 6803 by homologous recombination. Contained on the plasmid are left- and right flanking sequences obtained from the Synechocystis genome. These flanking sequences undergo homologous with their sister sequences in the genome of Synechocystis, integrating the plasmid sequence into the genome.

The reporter plasmid will use a fluorescent protein placed under the control of an indicuble promoter. This will allow us to easily verify that the inducible expression system works as intended inside Synechocystis sp. PCC 6803.

CO₂ fixating BioBrick

After verifying that the expression system is working as intended in Synechocystis, we will replace the fluorescent protein gene with one that is predicted to increase the rate of carbon fixation in the organism. The way we will idenfity this gene is by use of metabolic modelling. Once a candidate gene has been detected, it will be cloned and transformed into Synechocystis.