Team:Cambridge-JIC/Guide/Materials/Media

From 2014.igem.org

- Agrobacterium plates Use standard LB + 1.5% agar + required antibiotics. - Marchantia plates For marchantia, 1/2 Gamborg's B5 media + 1.2% agar is effective. Gamborg's B5 salts can be found readily from chemical suppliers.

Always use freshly autoclaved media, and work in a laminar flow hood, maintaining sterile conditions.

Fungal spores rapidly colonise the plates under conditions for Marchantia growth.

Always seal all plates using micropore tape. - Selection plates If using the Marchantia polymorpha resistance cassette and agrobacterium mediated transformation, use Marchantia plates with 10ug/ml hygromycin and 100ug/ml cefotaxime (to ensure that agrobacterium does not colonize the plate).

Note: selection plates do not contain a high enough concentration of hygromycin to preclude fungal colonisation. Work in sterile conditions.

- Acetosyringone Use a fresh stock, with a concentration of 100mM, in DMSO. This will be used in transformation protocols as 1000x.