The Arizona State University iGEM team
Current system of biodiesel production in E. coli
When attempting to produce biodesiel in E. Coli, certain problems emerge, especially surrounding the metabolic use of certain intermediate products. E. coli has to use the pyruvate from glycolysis to produce both intermediates of wax ethyl ester, ethanol and acyl-COAs. In order to circumvent this metabolic competition, we decided that specialization
Future plans for this project
There are some future objectives that can expand upon this project. Maximizing the production of ethanol and acyl-COAs without stopping normal cellular metabolism can optimize the production of the fatty ethyl esters. Another consideration is the balance between the ethanol and fatty acid populations. It is possible that more of one population is required to optimize production. Quorum sensing techniques could be used to track the two populations and reach an equilibrium with high production. |
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