Team:NTNU Trondheim/Project
From 2014.igem.org
WikitemplateB project
From 2014.igem.org
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Team Example's Project name! | ||||
Project IntroductionCO2 emissions have recieved a lot of attention in modern times, due to concerns that high emission levels are facilitating global warming. Consequently, a lot of research is focused on ways of reducing CO2 emissions from industry, and ways of fixating atmospheric CO at a greater than normal rate. Our project is attempting to produce a BioBrick, which when placed inside photosynthetic bacteria, increases their rate of CO2 fixation. In order to achieve this, we first need to construct a BioBrick that allows inducible expression of non native genes in our chassis; |
Results
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Reporter BioBrick | CO2 fixation BioBrick | Testing CO2 fixation rate |
Reporter BioBrickThe first stage of this project consists of creating a reporter BioBrick in order to confirm that we have are able to induce the expression of foreign genes in Once the reporter plasmid has been successfully constructed, we will verify that it is working as intended by inducing the expression of a fluorescent protein contained on the plasmid. Once induced, the colonies of cells transformed with this plasmid should exhibit a color that is determined by the fluorescent protein. |
The reporter plasmid will use a fluorescent protein placed under the control of an indicuble promoter. This will allow us to easily verify that the plasmid works as intended inside |