Team:DTU-Denmark/Achievements/Experimental Results

Since we introduced a mutation in the Spinach2 sequence to overcome a SpeI restriction site, our first task was to confirm that this modified Spinach2.1 was performing compatible to Spinach2. We met some complications when generating the spinach RNA by in vitro transcription. This can be due to different parameters ie. the instability of RNA and presence of RNAses. We therefore chose to use all the generated RNA to have the best foundation for measuring fluorescence. This is why Spinach2 and Spinach2.1 is not used in identical concentrations.

The RNA concentrations were measured:

• Spinach2: 40 nM/µl
Spinach2.1:

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